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mouse antimap2  (Thermo Fisher)


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Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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  • 90

    Structured Review

    Thermo Fisher mouse antimap2
    Mouse Antimap2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antimap2/anti+map2/pm38915059-184-49-52
    Average 90 stars, based on 1 article reviews
    mouse antimap2 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Astrocytic stress response is induced by exposure to astrocyte-binding antibodies expressed by plasmablasts from pediatric patients with acute transverse myelitis.
    Article Snippet: Slides were washed 2 × 5 min with PBS, then with 0.2% Triton X-100 (Sigma) for 10 min. .. Slides were blocked for 2 h with 5% Goat Serum (ThermoFisher 50062Z) + 0.1% Triton X-100 in a humidified chamber, then incubated overnight at 4C in a humidified chamber with primary antibodies diluted in blocking buffer: rhAbs (20 μg/mL), purified human IgG (2 μg/mL), Rabbit anti-GFAP (1:100 Abcam ab16997), Mouse antiMAP2 (1:200 ThermoFisher MA5-12826). .. The next day, slides were washed 3 × 5 min with 0.025% Triton X-100, then incubated for 1 h at RT in a humidified chamber with secondary antibodies diluted in blocking buffer: Goat anti-Human conj AlexaFluor 488 (1:1000 ThermoFisher A-11013), Goat anti-Rabbit conj AlexaFluor 568 (1:1000 Abcam ab175471), Goat anti-Mouse conj AlexaFluor 647 (1:500 Abcam ab150115).

    Blocking Assay:

    Article Title: Astrocytic stress response is induced by exposure to astrocyte-binding antibodies expressed by plasmablasts from pediatric patients with acute transverse myelitis.
    Article Snippet: Slides were washed 2 × 5 min with PBS, then with 0.2% Triton X-100 (Sigma) for 10 min. .. Slides were blocked for 2 h with 5% Goat Serum (ThermoFisher 50062Z) + 0.1% Triton X-100 in a humidified chamber, then incubated overnight at 4C in a humidified chamber with primary antibodies diluted in blocking buffer: rhAbs (20 μg/mL), purified human IgG (2 μg/mL), Rabbit anti-GFAP (1:100 Abcam ab16997), Mouse antiMAP2 (1:200 ThermoFisher MA5-12826). .. The next day, slides were washed 3 × 5 min with 0.025% Triton X-100, then incubated for 1 h at RT in a humidified chamber with secondary antibodies diluted in blocking buffer: Goat anti-Human conj AlexaFluor 488 (1:1000 ThermoFisher A-11013), Goat anti-Rabbit conj AlexaFluor 568 (1:1000 Abcam ab175471), Goat anti-Mouse conj AlexaFluor 647 (1:500 Abcam ab150115).

    Purification:

    Article Title: Astrocytic stress response is induced by exposure to astrocyte-binding antibodies expressed by plasmablasts from pediatric patients with acute transverse myelitis.
    Article Snippet: Slides were washed 2 × 5 min with PBS, then with 0.2% Triton X-100 (Sigma) for 10 min. .. Slides were blocked for 2 h with 5% Goat Serum (ThermoFisher 50062Z) + 0.1% Triton X-100 in a humidified chamber, then incubated overnight at 4C in a humidified chamber with primary antibodies diluted in blocking buffer: rhAbs (20 μg/mL), purified human IgG (2 μg/mL), Rabbit anti-GFAP (1:100 Abcam ab16997), Mouse antiMAP2 (1:200 ThermoFisher MA5-12826). .. The next day, slides were washed 3 × 5 min with 0.025% Triton X-100, then incubated for 1 h at RT in a humidified chamber with secondary antibodies diluted in blocking buffer: Goat anti-Human conj AlexaFluor 488 (1:1000 ThermoFisher A-11013), Goat anti-Rabbit conj AlexaFluor 568 (1:1000 Abcam ab175471), Goat anti-Mouse conj AlexaFluor 647 (1:500 Abcam ab150115).



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